HSC Research Associate - PI Dr. Gangwani

Extended Job Title
HSC Research Associate - PI Dr. Gangwani

Org Level 1
Texas Tech Unv Hlth Sci Ctr El Paso

Position Description
Performs specialized work in the planning, conducting and/or supervising of original research at TTUHSC. Responsible for participating in, planning, scheduling and/or supervising research projects and the interpretation of the results for publication. Work is performed under general supervision with evaluation based on accomplishment of assigned objectives and overall effectiveness of project. May supervise research and student assistants.

Requisition ID
23036BR

Travel Required
Up to 25%

Major/Essential Functions

• Performs staining of cells/tissues for immunofluorescence analysis and digital imaging of stained cells and tissues using microscopes, including confocal microscope.
• Biochemical assays and methods, including immunoprecipitation followed by Mass spectroscopy and automated western blot analyses to examine protein-protein interaction or protein-nucleic acids interaction in vitro with RNA-DNA hybrids and in vivo with R-loops. Effect of nucleotides such as ATP and GTP hydrolysis on resolution RNA-DNA hybrids.
• Participate in research data collection by performing highly skilled experiments using mammalian and yeast cells, including cultured primary fibroblast and neurons. Cell transfection and infection with siRNA, antisense oligos, plasmids and viral vectors. Perform recombinant DNA techniques (such as cloning, PCR amplification), isolation of nucleic acids, including genomic and plasmid DNAs, and proteins, including recombinant proteins from cells (bacteria, yeast or mammalian cells) for biochemical and molecular analysis.
• Breeding of animals for desired genotypes and genotyping of mice using PCR and southern blotting. Necropsy, tissue processing histology.
• Protein isolation, purification, identification, and quantitation using automated SDS-PAGE or western blot analysis.
• Mutational analysis using site directed mutagenesis. Cloning of genomic and cDNA for ectopic expression of genes at protein and mRNA levels, and designing of targeting vectors for genetic manipulation in cells and animals CRISPR and conventional methods. Nucleic acids sequencing.
• Collect and maintain a prudent record of all experiments performed. Prepare scientific data, carry out data analysis and prepare manuscripts for publication. Participation and presentation of data in weekly lab meetings.
• Responsible for day-to-day maintenance of lab and lab equipment, including ordering supplies, washing glassware, preparing reagents, etc.
• Guides and directs junior technicians and students.
• Other duties are required to ensure the smooth operation of the research lab and to facilitate the conduct of research.

Position Specific Qualifications
This position requires an engagement in an advanced level of scientific, technical and research related administrative work in support of current and future research projects in this laboratory. The candidate will have extensive knowledge of biomedical research, data collection, and documentation following research protocols and guidelines: developing and evaluating new research techniques and methods, including tissue culture of mammalian cell lines, including cells derived from human patients and primary cells, including spinal cord motor neurons from mice. Staining of cells and neurons with antibodies for immunofluorescence analysis using confocal microscopy. Experience in protein isolation, purification, quantitation, immunoprecipitation, separation using automated western blot analysis; isolation, purification, quantitation, time PCR (RT-PCR) base methods. Site directed mutagenesis, cloning and sequencing of nucleic acids. Histological and immunocytochemical (IHC) analysis of human and mice tissues. In addition must have experience breeding genetically modified animals for maintaining mice colonies and genotyping of mice using PCR; analysis of phenotypes of mice and collection of data. Analysis of expression of genes and proteins; Maintenance of accurate and up-to-date lab records and preparation of data presentations and scientific publications.


Grant Funded?
Yes

Minimum Hire Rate
15.64

Pay Basis
Hourly

Work Location
El Paso

Preferred Qualifications
The ideal candidate will: Have a Master's degree in the general area of biomedical sciences with knowledge and some expertise in the fields of cell biology and neurosciences, including neurological disorders. Prior experience (1 to 2 years) working with related laboratory research methods for conducting biomedical research, including confocal microscopy. Have 1 to 2 years of experience and skills working with laboratory research animals, including breeding and genotyping of genetically modified mouse models or willing to work with laboratory research animals. Evidence of having 1 to 2 years of experience in independent supervisory and organizational skills required for biomedical research laboratory. Experience using research related software, including Microsoft Excel, Graphpad Prism, NIH ImageJ, Abode Illustrator, and Photoshop.

Campus
HSC - El Paso

Department
Ctr of Exc Neurosciences Elp

Required Attachments
Cover Letter, Resume / CV

Job Type
Full Time

Pay Statement
Compensation is commensurate upon the qualifications of the individual selected and budgetary guidelines of the hiring department, as well as, the institutional pay plan. For additional information, please reference the institutional pay plan on the Human Resources webpage.

Job Group
Research Staff

Shift
Day

EEO Statement
As an EEO/AA employer, the Texas Tech University System and its components will not discriminate in our employment practices based on an applicants race, ethnicity, color, religion, sex, sexual orientation, gender identity, national origin, age, disability, genetic information or status as a protected veteran.

Required Qualifications
Master's degree or specialized academic training beyond the Bachelor's level in a particular field of science or engineering equivalent to a Master's degree. One year research experience. Knowledge of modern research practices, the methods, resources, and standards thereof.

Does this position work in a research laboratory?
Yes

Are select agents used in this laboratory?
No